Sterilization Equipment Validation Checklist

Ensure compliance & patient safety! Download our comprehensive Sterilization Equipment Validation Checklist for Facility Management. Validate autoclaves, sterilizers & more - streamline audits, reduce risk & maintain operational excellence. Free checklist download!

This Template was installed 1 times.

Document Control & Planning

1 of 12

Ensuring proper documentation and planning is in place for the validation process.

Validation Protocol Number

Protocol Creation Date

Protocol Review Date

Document Status (Draft/Approved/Revoked)

Document Scope & Objectives

Approved Protocol Document (PDF)

Validation Phase (Planning/Execution/Reporting)

Deviation Handling Procedure Reference

Protocol Approver Signature

Equipment Identification & Description

2 of 12

Clearly identifying the sterilization equipment being validated and documenting its key characteristics.

Equipment Name

Manufacturer

Model Number

Serial Number

Detailed Equipment Description (including configuration)

Capacity (e.g., number of trays)

Cycle Type(s) Validated (e.g., Gravity, Steam Flush, Vacuum)

Equipment Photos/Diagrams

Any known modifications to the standard equipment configuration

Personnel & Training

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Verification of personnel involved in the validation process are appropriately trained and qualified.

Number of Personnel Involved in Validation

Validation Personnel Roles

Brief Description of Personnel Training (e.g., manufacturer training, SOP review)

Date of Personnel Training

Attach Training Certificates/Records

Competency Assessment Performed?

Details of Competency Assessment (if applicable)

Signature of Validation Personnel (Confirmation of Training and Competency)

Validation Protocol Development

4 of 12

Review and approval of the validation protocol including acceptance criteria.

Protocol Purpose and Scope

Reference Documents (e.g., SOPs, Manufacturer's Instructions)

Number of Validation Runs

Validation Method (e.g., Release, Non-release)

Types of Indicators Used (e.g., Biological, Chemical)

Date Protocol Prepared

Acceptance Criteria – Sterility Assurance Level (SAL)

Specify 'Other' SAL, if applicable

Prepared By

Pre-Validation Equipment Checks

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Initial equipment checks to ensure proper function before commencing validation runs.

Equipment Serial Number

Date of Last Preventative Maintenance

Time of Equipment Startup for Pre-Validation Check

Voltage Reading (Input)

Pressure Reading (Initial)

Equipment Cycle Type (e.g., Gravity, Steam Flush, Vacuum)

Description of Pre-Validation Checks Performed

Visual Inspection - Equipment Condition

Technician Signature - Pre-Validation Check Completion

Validation Runs – Biological Indicators (BIs)

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Documentation and results of validation runs using biological indicators to assess sterilization efficacy.

BI Lot Number

Date of BI Exposure

Time of BI Exposure

BI Incubation Temperature (°C)

BI Incubation Time (hours)

BI Result – Positive/Negative

BI Result Comments (e.g., unusual growth, etc.)

Upload BI Result Image/Documentation

BI Type (e.g., Spore Disc, Strip)

Validation Runs – Chemical Indicators (CIs)

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Documentation and results of validation runs using chemical indicators to verify process parameters.

Cycle Number

Chemical Indicator Type Used (e.g., MSD, Dye, Integrator)

If 'Other' indicator type selected, please specify:

Number of Chemical Indicators Used

Indicator Placement (e.g., Inside, Outside, Top, Bottom)

If 'Other' indicator placement selected, please specify:

Time Indicator Observed (Initial)

Time Indicator Observed (Final)

Photo/Scan of Chemical Indicator Post-Cycle

Indicator Result (Based on Manufacturer Instructions)

Data Analysis & Evaluation

8 of 12

Review and analysis of data collected during validation runs to determine compliance with acceptance criteria.

BI Spore Count (CFU/mL)

Incubation Temperature (°C)

Incubation Time (hours)

BI Result (Positive/Negative)

CI Result (Passed/Failed)

Summary of Data Analysis

Acceptance Criteria Met?

Detailed Explanation of Results and Justification (if applicable)

Attach Raw Data Files (e.g., data logs, instrument printouts)

Deviation Handling & Corrective Actions

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Documentation of any deviations from the validation protocol and the implementation of corrective actions.

Description of Deviation

Severity of Deviation (Minor, Moderate, Major)

Root Cause Analysis

Corrective Action(s) Implemented

Date Corrective Action Implemented

Effectiveness Verification of Corrective Action

Verification Status (Satisfactory, Unsatisfactory)

Signature of Person Implementing Corrective Action

Validation Report & Documentation

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Preparation and review of the final validation report, including all supporting documentation.

Report Summary & Conclusion

Validation Protocol Document

Raw Data Files (BI & CI Results)

Number of Validation Cycles Completed

Deviations Encountered (Select all that apply)

Detailed Description of Deviations & Corrective Actions (If Applicable)

Report Completion Date

Validation Lead Signature

Report Approval Status

Equipment Relocation/Maintenance/Repair Validation

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Validation performed after equipment relocation, significant maintenance, or repair.

Description of Relocation/Maintenance/Repair Performed

Reason for Relocation/Maintenance/Repair

Date of Relocation/Maintenance/Repair

Time of Relocation/Maintenance/Repair (Start)

Cycle Number on Which Validation was Performed

Pre-Relocation/Maintenance/Repair Equipment Log/Service Records

Software Version (if applicable)

Detailed Description of Post-Maintenance/Repair Checks

Technician Signature - Verification of Work Completion

Periodic Revalidation

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Documentation of periodic revalidation activities to ensure continued sterilization effectiveness.

Date of Revalidation

Reason for Revalidation (e.g., Time-based, Equipment Modification, Deviation)

Brief Description of Revalidation Activities Performed

Biological Indicator (BI) Records

Chemical Indicator (CI) Records

Number of BI Strips Used

BI Results: All Positive/Negative?

Explanation/Details of any Deviation from Expected Results (if applicable)

Reviewer Signature

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