Laboratory Safety Inspection Checklist (Science Labs)

Ensure a safe and compliant science lab! Download our comprehensive Laboratory Safety Inspection Checklist, part of our Facility Management series. Identify hazards, meet regulatory requirements, and protect your students and staff - all with this easy-to-use checklist.

This Template was installed 3 times.

General Facility Conditions

1 of 10

Assessment of overall lab cleanliness, organization, and structural integrity.

Ambient Temperature (°C)

Noise Level (dB)

Floor Condition (Overall)

Wall & Ceiling Condition

Notes on General Cleanliness & Organization

Presence of Pests?

Photos of General Facility Condition (Optional)

Ventilation & Air Quality

2 of 10

Evaluation of ventilation systems and air quality control measures.

Room Air Changes per Hour (ACH)

Fume Hood Certification Status?

Describe any unusual odors or ventilation concerns observed.

Local Exhaust Ventilation (LEV) Functionality

Fume Hood Face Velocity (m/s or ft/min)

Which Ventilation System Components are visible? (Check all that apply)

Are Ventilation Systems Regularly Maintained?

Lighting & Electrical Safety

3 of 10

Review of lighting adequacy and electrical hazards.

Ambient Light Level (Lux)

Lighting Condition

Electrical Hazards Observed

GFCIs Tested?

Circuit Breaker Amp Rating (Check against equipment draw)

Comments/Notes on Lighting and Electrical Safety

Emergency Equipment & Accessibility

4 of 10

Inspection of safety showers, eyewash stations, fire extinguishers, and first aid kits – ensuring they are functional and accessible.

Last Eyewash Station Inspection Date

Last Safety Shower Inspection Date

Number of Functioning Fire Extinguishers

Eyewash Station Water Pressure (Adequate/Insufficient)

Safety Shower Water Temperature (Acceptable/Too Hot/Too Cold)

Fire Extinguisher Inspection Tags Present and Valid?

Comments/Observations Regarding Emergency Equipment

Location of First Aid Kit

Hazardous Materials Storage

5 of 10

Verification of proper storage practices for chemicals, biological agents, and other hazardous materials.

Temperature of Flammable Storage Refrigerator

Chemical Cabinets Securely Fastened to Wall?

Incompatible Chemicals Segregated?

Secondary Containment Used for Liquids?

Comments on Chemical Storage Practices

Date of last chemical inventory

Compressed Gas Cylinders Properly Secured?

Hazardous Waste Management

6 of 10

Review of waste disposal procedures and container labeling.

Number of Hazardous Waste Containers

Waste Segregation Practices

Container Label Status

Date of Last Waste Pickup

Notes on Waste Management Practices (if any)

Waste Manifests are Properly Maintained

Upload Photo of Waste Storage Area

Personal Protective Equipment (PPE)

7 of 10

Assessment of PPE availability, condition, and proper usage protocols.

Are appropriate PPE readily available to all lab personnel?

Which types of PPE are typically required in this lab?

If gloves are required, what type(s) are appropriate? (e.g., Nitrile, Latex, etc.)

Are employees adequately trained on proper PPE selection and usage?

Number of available lab coats.

Any observations regarding the condition of PPE (e.g., damaged lab coats, mismatched gloves)?

Are designated areas for PPE storage clearly marked?

Emergency Procedures & Signage

8 of 10

Evaluation of emergency plans, evacuation routes, and safety signage.

Are evacuation maps posted in prominent locations?

Are emergency contact numbers clearly posted?

Date of last fire drill (MM/DD/YYYY)

Briefly describe any observed deficiencies related to emergency signage.

Check all that apply regarding emergency procedures.

Are emergency exits clearly marked and unobstructed?

Date of last emergency procedure review

Structural Integrity & Housekeeping

9 of 10

Inspection of flooring, walls, ceilings, and overall housekeeping practices.

Floor Condition Rating (1-5, 1=Excellent, 5=Poor)

Floor Damage Observed (Check all that apply)

Describe any wall or ceiling damage observed.

Evidence of Pests (Rodents, Insects)?

Dust Accumulation on Surfaces (Scale of 1-5, 1=None, 5=Excessive)

Are Aisles and Pathways Clear and Unobstructed?

Describe any housekeeping deficiencies observed.

Laboratory Specific Hazards

10 of 10

Assessment of unique hazards related to specific laboratory activities and equipment.

Describe any unique experimental procedures carried out in the lab and potential associated hazards.

Are any specialized equipment (e.g., autoclaves, fume hoods, centrifuges) in use? Select all that apply.

If 'Other' was selected for specialized equipment, please specify.

For procedures involving radioactivity, record the last date of calibration/inspection of radiation monitoring equipment.

For labs working with biological agents, has a risk assessment been conducted for each agent?

Date of last biological agent risk assessment review (if applicable)

Location of any specifically hazardous materials (e.g., concentrated acids, strong oxidizers)

Upload relevant Standard Operating Procedures (SOPs) for unique processes

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