Dental Sterilization Checklist: Equipment & Procedure Validation

Ensure patient safety and regulatory compliance with our comprehensive Dental Sterilization Checklist! Validate equipment performance, confirm procedural accuracy, and minimize infection risk. Download now for a safer, more efficient dental practice.

This Template was installed 2 times.

Pre-Cleaning & Preparation

1 of 8

Initial steps before sterilization, including rinsing, scrubbing, and disassembly.

Date of Pre-Cleaning

Time of Initial Rinse

Description of Visible Debris (e.g., blood, saliva)

Rinsing Solution Used

Rinsing Time (minutes)

Contaminants Removed

Notes on Instrument Condition

Loading the Autoclave/Sterilizer

2 of 8

Ensuring proper loading techniques for optimal sterilization, including tray packing and air removal.

Tray Load Capacity (Max)

Load Type

Tray Orientation

Air Removal Method

Cycle Number/ID (if applicable)

Special Instructions/Notes for Load

Packaging Type

Cycle Parameters Verification

3 of 8

Confirming correct temperature, pressure, and time settings for the sterilization cycle.

Temperature (°C)

Pressure (PSI)

Cycle Time (minutes)

Cycle Type

Load Type

Cycle Completion Date

Cycle Start Time

Autoclave/Sterilizer Maintenance

4 of 8

Routine checks, cleaning, and maintenance of the sterilizer equipment.

Last Preventative Maintenance Date

Water Reservoir Level (inches)

Notes on Visual Inspection (e.g., corrosion, leaks)

Air Pump Pressure (PSI)

Filter Replacement Status

Next Scheduled Maintenance Date

Any unusual sounds during cycle?

Biological Indicator Testing

5 of 8

Validation of sterilization effectiveness using biological indicators (e.g., spore tests).

Date of Biological Indicator Test

Time of Biological Indicator Test

Type of Biological Indicator

Lot Number of Biological Indicator

Incubation Temperature (°C)

Notes/Observations during incubation (e.g., unusual growth)

Result

Image of Biological Indicator (if applicable)

Signature of Person Performing Test

Chemical Indicator Monitoring

6 of 8

Checking for proper sterilization using chemical indicators on each load.

Indicator Type (e.g., MSD, Bowie-Dick)

Indicator Result

Lot Number/Batch ID (if applicable)

Date of Monitoring

Time of Monitoring

Comments/Observations (e.g., discoloration, reading)

Photo of Indicator (optional)

Loading and Unloading Procedures

7 of 8

Safe handling and segregation of sterilized and non-sterilized instruments.

Number of Instrument Trays Loaded

Instrument Tray Orientation (Check all that apply)

Air Removal Method

Time of Unloading

Notes on Loading/Unloading Process (e.g., unusual observations)

Packaging Type (for Unloading)

Storage Location of Sterilized Instruments

Record Keeping & Documentation

8 of 8

Maintaining accurate records of sterilization cycles, indicator results, and equipment maintenance.

Cycle Date

Cycle Start Time

Cycle Number

Sterilizer Type

Biological Indicator Result

Biological Indicator Report (Optional)

Notes/Comments

Operator Signature

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